Atomic structure of a nanobody-trapped domain-swapped dimer of an amyloidogenic β2-microglobulin variant
نویسندگان
چکیده
Department of Molecular and Cellular Interactions, Vlaams Instituut voor Biotechnologie, Pleinlaan 2, B-1050 Brussels, Belgium; Structural Biology Brussels, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussels, Belgium; Grenoble Outstation, European Molecular Biology Laboratory and Unit of Virus Host-Cell Interactions, 6 rue Jules Horowitz, BP181, 38042 Grenoble Cedex 9, France; and Department of Biochemistry, University of Pavia, Via Taramelli 3b, 27100 Pavia, Italy
منابع مشابه
Atomic structure of a nanobody-trapped domain-swapped dimer of an amyloidogenic beta2-microglobulin variant.
Atomic-level structural investigation of the key conformational intermediates of amyloidogenesis remains a challenge. Here we demonstrate the utility of nanobodies to trap and characterize intermediates of β2-microglobulin (β2m) amyloidogenesis by X-ray crystallography. For this purpose, we selected five single domain antibodies that block the fibrillogenesis of a proteolytic amyloidogenic frag...
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